2026
Chen, G.; Wang, H.; Straus, S.; Li, H. The Minimal Folding Motif of the Repeat-in-Toxin Domain of Adenylate Cyclase Toxin. Langmuir 2026, 42, 4, 3584-3593.
2025
Li, H.; Bian, Q. Stimuli-responsive protein hydrogels: From dynamic tuning of hydrogel mechanics to shape morphing. Supramolecular Materials 2025, 100120.
Bian, Q.; Li, H. Fully Reshapeable and Recyclable Protein Hydrogels. ADVANCED MATERIALS 2025, 37(42), e09812.
Fu, L. ; Li, H. Ionic Crosslinking Improves the Stiffness and Toughness of Protein Hydrogels. Polymer Science & Technology 2025, 1(4), 342-350.
Zuo, J.; Li. H. Intermolecular Misfolding Captured in Parallelly Organized Titin. Journal of the American Chemical Society, 2025, 147(6), 4853-4861.
2024
Gao, X. ; Chen, Y. ; Li, H. ; Hu, C. Measurement Of Rotated Janus Particle’s Position And Orientation In The Optical Trap. NANOTECHNOLOGY AND PRECISION ENGINEERING 2024, 7, 043001.
Li, P. ; Li, H. A Handle-Free, All-Protein-Based Optical Tweezers Method To Probe Protein Folding-Unfolding Dynamics. Langmuir 2024, 40, 13721-13727.
Liu, X. ; Yao, R. ; Sun, Z. ; Zhang, H. ; Yan, F. ; Li, H. ; Zhang, W. Single-Molecule Study Reveals Ion-Dependent Conformational Change And Nanomechanical Property Of Crown Ether-Based Polymer. Macromolecules 2024, 57, 3798-3806.
Tubiana, L. ; Alexander, G. P. ; Barbensi, A. ; Buck, D. ; Cartwright, J. H. E. ; Chwastyk, M. ; Cieplak, M. ; Coluzza, I. ; Copar, S. ; Craik, D. J. ; Di Stefano, M. ; Everaers, R. ; Faisca, P. F. N. ; Ferrari, F. ; Giacometti, A. ; Goundaroulis, D. ; Haglund, E. ; Hou, Y. – M. ; Ilieva, N. ; Jackson, S. E. ; Japaridze, A. ; Kaplan, N. ; Klotz, A. R. ; Li, H. ; Likos, C. N. ; Locatelli, E. ; Lopez-Leon, T. ; Machon, T. ; Micheletti, C. ; Michieletto, D. ; Niemi, A. ; Niemyska, W. ; Niewieczerzal, S. ; Nitti, F. ; Orlandini, E. ; Pasquali, S. ; Perlinska, A. P. ; Podgornik, R. ; Potestio, R. ; Pugno, N. M. ; Ravnik, M. ; Ricca, R. ; Rohwer, C. M. ; Rosa, A. ; Smrek, J. ; Souslov, A. ; Stasiak, A. ; Steer, D. ; Sulkowska, J. ; Sulkowski, P. ; Sumners, D. Witt L. ; Svaneborg, C. ; Szymczak, P. ; Tarenzi, T. ; Travasso, R. ; Virnau, P. ; Vlassopoulos, D. ; Ziherl, P. ; Zumer, S. Topology In Soft And Biological Matter. PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS 2024, 1075, 1-137.
Bian, Q. ; Kong, N. ; Arslan, S. ; Li, H. Calmodulin-Based Dynamic Protein Hydrogels With Three Distinct Mechanical Stiffness. ADVANCED FUNCTIONAL MATERIALS 2024, 34.
Lei, J. ; Li, H. The Photocleavable Protein Phocl-Based Dynamic Hydrogels. ACS BIOMATERIALS SCIENCE & ENGINEERING 2024, 10, 7404-7412
2023
Wang, H.; Miao, X.; Zhai, C.; Chen, Y.; Lin, Z.; Zhou, X.; Guo, M.; Chai, Z.; Wang, R.; Shen, W.; Li, H.; Hu, C. Mechanistic Insights into the Folding Mechanism of Region v in Ice-Binding Protein Secreted by Marinomonas Primoryensis Revealed by Single-Molecule Force Spectroscopy. Langmuir 2023, 39 (45), 16128–16137.
Chen, G.; Wang, H.; Bumba, L.; Mašín, J.; Šebo, P.; Li, H. The Adenylate Cyclase Toxin RTX Domain Follows a Series Templated Folding Mechanism with Implications for Toxin Activity. Journal of Biological Chemistry 2023, 299 (9), 105150–105150.
Fu, L.; Li, L.; Bian, Q.; Xue, B.; Jin, J.; Li, J.; Cao, Y.; Jiang, Q.; Li, H. Cartilage-like Protein Hydrogels Engineered via Entanglement. Nature 2023, 618 (7966), 740–747.
Zuo, J.; Chen, H.; Li, H. Two Molecule Force Spectroscopy on Ligand-Receptor Interactions. Nanoscale 2023, 15 (41), 16581–16589.
Li, H. Single Molecule Force Spectroscopy Studies on Metalloproteins: Opportunities and Challenges. Langmuir 2023, 39 (4), 1345–1353.
Zuo, J.; Li, H. Two-Molecule Force Spectroscopy on Proteins. ACS Nano 2023, 17 (8), 7872–7880.
2022
Liu, J.; Guo, H.; Gao, Q.; Li, H.; An, Z.; Zhang, W. Coil–Globule Transition of a Water-Soluble Polymer. Macromolecules 2022, 55 (19), 8524–8532.
Duan, T.; Bian, Q.; Li, H. Protein Hydrogels with Reversibly Patterned Multidimensional Fluorescent Images for Information Storage. Biomacromolecules 2022, 23 (7), 3009–3016.
Wang, H.; Chen, G.; Li, H. Templated Folding of the RTX Domain of the Bacterial Toxin Adenylate Cyclase Revealed by Single Molecule Force Spectroscopy. Nature Communications 2022, 13 (1).
Gao, X.; Zhai, C.; Lin, Z.; Chen, Y.; Li, H.; Hu, C. Simulation and Experiment of the Trapping Trajectory for Janus Particles in Linearly Polarized Optical Traps. Micromachines 2022, 13 (4), 608.
Bian, Q.; Fu, L.; Li, H. Engineering Shape Memory and Morphing Protein Hydrogels Based on Protein Unfolding and Folding. Nature Communications 2022, 13 (1).
Xia, J.; Zuo, J.; Li, H. Molecular Homogeneity of GB1 Revealed by Single Molecule Force Spectroscopy. Nanoscale 2022, 14 (27), 9925–9931
Li, J.; Li, H. New Insights into the Folding–Unfolding Mechanism and Conformations of Cytochrome C. Chemical Science 2022, 13 (25), 7498–7508.
2021
Zuo, J. ; Zhan, D. ; Xia, J. ; Li, H. Single-Molecule Force Spectroscopy Studies Of Missense Titin Mutations That Are Likely Causing Cardiomyopathy. LANGMUIR 2021, 37, 12128-12137.
Li, J. ; Chen, G. ; Guo, Y. ; Wang, H. ; Li, H. Single Molecule Force Spectroscopy Reveals The Context Dependent Folding Pathway Of The C-Terminal Fragment Of Top7. CHEMICAL SCIENCE 2021, 12, 2876-2884.
Duan, T. ; Bian, Q. ; Li, H. Light-Responsive Dynamic Protein Hydrogels Based On Lovtrap. LANGMUIR 2021, 37, 10214-10222.
Li, H. There Is Plenty Of Room In The Folded Globular Proteins: Tandem Modular Elastomeric Proteins Offer New Opportunities In Engineering Protein-Based Biomaterials. ADVANCED NANOBIOMED RESEARCH 2021, 1, 2100028.
Zhai, C. ; Hu, C. ; Li, S. ; Ma, Y. ; Zhang, Y. ; Guo, T. ; Li, H. ; Hu, X. The Formation Principle Of Micro-Droplets Induced By Using Optical Tweezers. NANOSCALE ADVANCES 2021, 3, 279-286.
Wang, R. ; Liu, M. ; Wang, H. ; Xia, J. ; Li, H. Gb Tags: Small Covalent Peptide Tags Based On Protein Fragment Reconstitution. BIOCONJUGATE CHEMISTRY 2021, 32, 1926-1934.
2020
Wang, H. ; Li, H. Mechanically Tightening, Untying And Retying A Protein Trefoil Knot By Single-Molecule Force Spectroscopy. CHEMICAL SCIENCE 2020, 11, 12512-12521.
Li, J. ; Li, H. Single Molecule Force Spectroscopy Reveals That A Two-Coordinate Ferric Site Is Critical For The Folding Of Holo-Rubredoxin. NANOSCALE 2020, 12, 22564-22573.
Fu, L. ; Li, H. Toward Quantitative Prediction Of The Mechanical Properties Of Tandem Modular Elastomeric Protein-Based Hydrogels. MACROMOLECULES 2020, 53, 4704-4710.
Ma, G. ; Hu, C. ; Li, S. ; Gao, X. ; Li, H. ; Hu, X. Axial Displacement Calibration And Tracking Of Optically Trapped Beads. OPTICS AND LASERS IN ENGINEERING 2020, 134, 106285.
Duan, T. ; Li, H. In Situ Phase Transition Of Elastin-Like Polypeptide Chains Regulates Thermoresponsive Properties Of Elastomeric Protein-Based Hydrogels. BIOMACROMOLECULES 2020, 21, 2258-2267.
Gao, X. ; Wang, Y. ; He, X. ; Xu, M. ; Zhu, J. ; Hu, X. ; Hu, X. ; Li, H. ; Hu, C. Angular Trapping Of Spherical Janus Particles. SMALL METHODS 2020, 4, 2000565.
Wang, Y. ; Wang, H. ; Tran, M. V. ; W. Algar, R. ; Li, H. Yellow Fluorescent Protein-Based Label-Free Tension Sensors For Monitoring Integrin Tension. CHEMICAL COMMUNICATIONS 2020, 56, 5556-5559.
Teixeira, M. H. ; Curtolo, F. ; Camilo, S. R. G. ; Field, M. J. ; Zheng, P. ; Li, H. ; Arantes, G. M. Modeling The Hydrolysis Of Iron-Sulfur Clusters. JOURNAL OF CHEMICAL INFORMATION AND MODELING 2020, 60, 653-660.
Mehlich, A. ; Fang, J. ; Pelz, B. ; Li, H. ; Stigler, J. Slow Transition Path Times Reveal A Complex Folding Barrier In A Designed Protein. FRONTIERS IN CHEMISTRY 2020, 8, 587824.
2019
Fu, L.; Haage, A.; Kong, N.; Tanentzapf, G.; Li, H. Dynamic Protein Hydrogels with Reversibly Tunable Stiffness Regulate Human Lung Fibroblast Spreading Reversibly. CHEMICAL COMMUNICATIONS 2019, 55, 5235–5238.
He, C.; Li, S.; Gao, X.; Xiao, A.; Hu, C.; Hu, X.; Hu, X.; Li, H. Direct Observation of the Fast and Robust Folding of a Slipknotted Protein by Optical Tweezers. NANOSCALE 2019, 11, 3945–3951.
Wang, H.; Gao, X.; Hu, X.; Hu, X.; Hu, C.; Li, H. Mechanical Unfolding and Folding of a Complex Slipknot Protein Probed by Using Optical Tweezers. BIOCHEMISTRY 2019, 58, 4751–4760.
Xia, J.; Zuo, J.; Li, H. Single Molecule Force Spectroscopy Reveals That the Oxidation State of Cobalt Ions Plays an Important Role in Enhancing the Mechanical Stability of Proteins. NANOSCALE 2019, 11, 19791–19796.
Li, S.; Hu, C.; Gao, X.; Ma, G.; Li, H.; Hu, X.; Hu, X. Optical Tweezers Assisted Controllable Formation and Precise Manipulation of Microdroplet. APPLIED PHYSICS EXPRESS 2019, 12, 117001.
Wang, H.; Gao, X.; Li, H. Single Molecule Force Spectroscopy Reveals the Mechanical Design Governing the Efficient Translocation of the Bacterial Toxin Protein Rtx. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2019, 141, 20498–20506.
Xiao, A.; Li, H. Direct Monitoring of Equilibrium Protein Folding-Unfolding by Atomic Force Microscopy: Pushing the Limit. CHEMICAL COMMUNICATIONS 2019, 55, 12920–12923.
Wang, R.; Li, J.; Li, X.; Guo, J.; Liu, J.; Li, H. Engineering Protein Polymers of Ultrahigh Molecular Weight via Supramolecular Polymerization: Towards Mimicking the Giant Muscle Protein Titin. CHEMICAL SCIENCE 2019, 10, 9277–9284.
Sun, W.; Duan, T.; Cao, Y.; Li, H. An Injectable Self-Healing Protein Hydrogel with Multiple Dissipation Modes and Tunable Dynamic Response. BIOMACROMOLECULES 2019, 20, 4199–4207.
Wang, R.; Fu, L.; Liu, J.; Li, H. Decorating Protein Hydrogels Reversibly Enables Dynamic Presentation and Release of Functional Protein Ligands on Protein Hydrogels. CHEMICAL COMMUNICATIONS 2019, 55, 12703–12706.
Lv, S.; Duan, T.; Li, H. Engineering Protein-Clay Nanosheets Composite Hydrogels with Designed Arginine-Rich Proteins. LANGMUIR 2019, 35, 7255–7260.
2018
Hu, C.; Su, C.; Yun, Z.; Wang, S.; He, C.; Gao, X.; Li, S.; Li, H.; Hu, X.; Hu, X. Real-Time Identification of the Singleness of a Trapped Bead in Optical Tweezers. APPLIED OPTICS 2018, 57, 1241–1246.
Li, J.; Li, H. Mechanical Unfolding Pathway of the High-Potential Iron-Sulfur Protein Revealed by Single-Molecule Atomic Force Microscopy: Toward a General Unfolding Mechanism for Iron-Sulfur Proteins. JOURNAL OF PHYSICAL CHEMISTRY B 2018, 122, 9340–9349.
Nassar, R.; Wong, E.; Bui, J. M.; Yip, C. K.; Li, H.; Gsponer, J.; Lamour, G. Mechanical Anisotropy in Gnnqqny Amyloid Crystals. JOURNAL OF PHYSICAL CHEMISTRY LETTERS 2018, 9, 4901–4909.
Li, H.; Zheng, P. Single Molecule Force Spectroscopy: A New Tool for Bioinorganic Chemistry. CURRENT OPINION IN CHEMICAL BIOLOGY 2018, 43, 58–67.
2017
Lyu, S.; Fang, J.; Duan, T.; Fu, L.; Liu, J.; Li, H. Optically Controlled Reversible Protein Hydrogels Based on Photoswitchable Fluorescent Protein Dronpa. CHEMICAL COMMUNICATIONS 2017, 53, 13375–13378.
Gao, X.; Lyu, S.; Li, H. Decorating a Blank Slate Protein Hydrogel: A General and Robust Approach for Functionalizing Protein Hydrogels. BIOMACROMOLECULES 2017, 18, 3726–3732.
Wang, R.; Qiao, S.; Zhao, L.; Hou, C.; Li, X.; Liu, Y.; Luo, Q.; Xu, J.; Li, H.; Liu, J. Dynamic Protein Self-Assembly Driven by Host-Guest Chemistry and the Folding-Unfolding Feature of a Mutually Exclusive Protein. CHEMICAL COMMUNICATIONS 2017, 53, 10532–10535.
Lei, H.; He, C.; Hu, C.; Li, J.; Hu, X.; Hu, X.; Li, H. Single-Molecule Force Spectroscopy Trajectories of a Single Protein and Its Polyproteins Are Equivalent: A Direct Experimental Validation Based on a Small Protein Nug2. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2017, 56, 6117–6121.
Harden, J. L.; Li, H. Editorial: Special Issue on Designer Protein Biomaterials. ACS BIOMATERIALS SCIENCE & ENGINEERING 2017, 3, 658–660.
Kong, N.; Fu, L.; Peng, Q.; Li, H. Metal Chelation Dynamically Regulates the Mechanical Properties of Engineered Protein Hydrogels. ACS BIOMATERIALS SCIENCE & ENGINEERING 2017, 3, 742–749.
Lamour, G.; Nassar, R.; Chan, P. H. W.; Bozkurt, G.; Li, J.; Bui, J. M.; Yip, C. K.; Mayor, T.; Li, H.; Wu, H.; Gsponer, J. A. Mapping the Broad Structural and Mechanical Properties of Amyloid Fibrils. BIOPHYSICAL JOURNAL 2017, 112, 584–594.
Lei, H.; Guo, Y.; Hu, X.; Hu, C.; Hu, X.; Li, H. Reversible Unfolding and Folding of the Metalloprotein Ferredoxin Revealed by Single-Molecule Atomic Force Microscopy. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2017, 139, 1538–1544.
He, C.; Li, H. Staphylokinase Displays Surprisingly Low Mechanical Stability. LANGMUIR 2017, 33, 1077–1083.
Li, H.; Walker, G. C. Twist and Shout: Single-Molecule Mechanochemistry. ACS NANO 2017, 11, 28–30.
2016
Gao, X.; Fang, J.; Xue, B.; Fu, L.; Li, H. Engineering Protein Hydrogels Using Spycatcher-Spytag Chemistry. BIOMACROMOLECULES 2016, 17, 2812–2819.
Li, H.; Kong, N.; Laver, B.; Liu, J. Hydrogels Constructed from Engineered Proteins. SMALL 2016, 12, 973–987.
Feng, J.; Lamour, G.; Xue, R.; Mirvakliki, M. N.; Hatzikiriakos, S. G.; Xu, J.; Li, H.; Wang, S.; Lu, X. Chemical, Physical and Morphological Properties of Bacterial Biofilms Affect Survival of Encased Campylobacter jejuni F38011 under Aerobic Stress. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 2016, 238, 172–182.
Si, C.; Li, J.; Luo, Q.; Hou, C.; Pan, T.; Li, H.; Liu, J. An Ion Signal Responsive Dynamic Protein Nano-Spring Constructed by High Ordered Host-Guest Recognition. CHEMICAL COMMUNICATIONS 2016, 52, 2924–2927.
2015
Zheng, P.; Arantes, G. M.; Field, M. J.; Li, H. Force-Induced Chemical Reactions on the Metal Centre in a Single Metalloprotein Molecule. NATURE COMMUNICATIONS 2015, 6, 7569.
Hu, C.; An, R.; Zhang, C.; Lei, H.; Hu, X.; Li, H.; Hu, X. Design of a High-Quality Optical Conjugate Structure in Optical Tweezers. APPLIED OPTICS 2015, 54, 1410–1413.
Lei, H.; Hu, X.; Zhu, P.; Chang, X.; Zeng, Y.; Hu, C.; Li, H.; Hu, X. Nano-Level Position Resolution for Particle Tracking in Digital In-Line Holographic Microscopy. JOURNAL OF MICROSCOPY 2015, 260, 106.
Kong, N.; Li, H. Protein Fragment Reconstitution as a Driving Force for Self-Assembling Reversible Protein Hydrogels. ADVANCED FUNCTIONAL MATERIALS 2015, 25, 5593–5601.
Panwar, P.; Lamour, G.; Mackenzie, N. C. W.; Yang, H.; Ko, F.; Li, H.; Broemme, D. Changes in Structural-Mechanical Properties and Degradability of Collagen during Aging-Associated Modifications. JOURNAL OF BIOLOGICAL CHEMISTRY 2015, 290, 23291–23306.
He, C.; Hu, C.; Hu, X.; Hu, X.; Xiao, A.; Perkins, T. T.; Li, H. Direct Observation of the Reversible Two-State Unfolding and Refolding of an Alpha/Beta Protein by Single-Molecule Atomic Force Microscopy. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2015, 54, 9921–9925.
2014
Zheng, P.; Wang, Y.; Li, H. Reversible Unfolding-Refolding of Rubredoxin: A Single-Molecule Force Spectroscopy Study. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2014, 53, 14060–14063.
Kong, N.; Peng, Q.; Li, H. Rationally Designed Dynamic Protein Hydrogels with Reversibly Tunable Mechanical Properties. ADVANCED FUNCTIONAL MATERIALS 2014, 24, 7310–7317.
Kovacic, S.; Samii, L.; Lamour, G.; Li, H.; Linke, H.; Bromley, E. H. C.; Woolfson, D. N.; Curmi, P. M. G.; Forde, N. R. Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. BIOMACROMOLECULES 2014, 15, 4065–4072.
Hu, X.; Li, H. Force Spectroscopy Studies on Protein-Ligand Interactions: A Single Protein Mechanics Perspective. FEBS LETTERS 2014, 588, 3613–3620.
He, C.; Lamour, G.; Xiao, A.; Gsponer, J.; Li, H. Mechanically Tightening a Protein Slipknot into a Trefoil Knot. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2014, 136, 11946–11955.
Xue, Y.; Li, X.; Li, H.; Zhang, W. Quantifying Thiol-Gold Interactions towards the Efficient Strength Control. NATURE COMMUNICATIONS 2014, 5, 4348.
Bull, M. S.; Sullan, R. M. A.; Li, H.; Perkins, T. T. Improved Single Molecule Force Spectroscopy Using Micromachined Cantilevers. ACS NANO 2014, 8, 4984–4995.
Lamour, G.; Yip, C. K.; Li, H.; Gsponer, J. High Intrinsic Mechanical Flexibility of Mouse Prion Nanofibrils Revealed by Measurements of Axial and Radial Young’s Moduli. ACS NANO 2014, 8, 3851–3861.
Wang, Y.; Hu, X.; Bu, T.; Hu, C.; Hu, X.; Li, H. Molecular Calipers for Highly Precise and Accurate Measurements of Single-Protein Mechanics. LANGMUIR 2014, 30, 2761–2767.
2013
Panwar, P.; Du, X.; Sharma, V.; Lamour, G.; Castro, M.; Li, H.; Broemme, D. Effects of Cysteine Proteases on the Structural and Mechanical Properties of Collagen Fibers. JOURNAL OF BIOLOGICAL CHEMISTRY 2013, 288, 5940–5950.
Fang, J.; Mehlich, A.; Koga, N.; Huang, J.; Koga, R.; Gao, X.; Hu, C.; Jin, C.; Rief, M.; Kast, J.; Baker, D.; Li, H. Forced Protein Unfolding Leads to Highly Elastic and Tough Protein Hydrogels. NATURE COMMUNICATIONS 2013, 4.
Zoldak, G.; Stigler, J.; Pelz, B.; Li, H.; Rief, M. Ultrafast Folding Kinetics and Cooperativity of Villin Headpiece in Single-Molecule Force Spectroscopy. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2013, 110, 18156–18161.
Ostapchenko, V. G.; Beraldo, F. H.; Mohammad, A. H.; Xie, Y.-F.; Hirata, P. H. F.; Magalhaes, A. C.; Lamour, G.; Li, H.; Maciejewski, A.; Belrose, J. C.; Teixeira, B. L.; Fahnestock, M.; Ferreira, S. T.; Cashman, N. R.; Hajj, G. N. M.; Jackson, M. F.; Choy, W.-Y.; MacDonald, J. F.; Martins, V. R.; Prado, V. F.; Prado, M. A. M. The Prion Protein Ligand, Stress-Inducible Phosphoprotein 1, Regulates Amyloid-Beta Oligomer Toxicity. JOURNAL OF NEUROSCIENCE 2013, 33, 16552–16564.
Bai, Y.; Luo, Q.; Zhang, W.; Miao, L.; Xu, J.; Li, H.; Liu, J. Highly Ordered Protein Nanorings Designed by Accurate Control of Glutathione S-Transferase Self-Assembly. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2013, 135, 10966–10969.
Zheng, P.; Takayama, S.-i. J.; Mauk, G. A.; Li, H. Single Molecule Force Spectroscopy Reveals That Iron Is Released from the Active Site of Rubredoxin by a Stochastic Mechanism. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2013, 135, 7992–8000.
Lv, S.; Bu, T.; Kayser, J.; Bausch, A.; Li, H. Towards Constructing Extracellular Matrix-Mimetic Hydrogels: An Elastic Hydrogel Constructed from Tandem Modular Proteins Containing Tenascin Fniii Domains. ACTA BIOMATERIALIA 2013, 9, 6481–6491.
2012 and before
Peng, Q.; Kong, N.; Wang, H.-C. E.; Li, H. Designing Redox Potential-Controlled Protein Switches Based on Mutually Exclusive Proteins. PROTEIN SCIENCE 2012, 21, 1222–1230.
Bu, T.; Wang, H.-C. E.; Li, H. Single Molecule Force Spectroscopy Reveals Critical Roles of Hydrophobic Core Packing in Determining the Mechanical Stability of Protein GB1. LANGMUIR 2012, 28, 12319–12325.
Shen, T.; Cao, Y.; Zhuang, S.; Li, H. Engineered Bi-Histidine Metal Chelation Sites Map the Structure of the Mechanical Unfolding Transition State of an Elastomeric Protein Domain GB1. BIOPHYSICAL JOURNAL 2012, 103, 807–816.
Fang, J.; Li, H. A Facile Way to Tune Mechanical Properties of Artificial Elastomeric Proteins-Based Hydrogels. LANGMUIR 2012, 28, 8260–8265.
He, C.; Genchev, G. Z.; Lu, H.; Li, H. Mechanically Untying a Protein Slipknot: Multiple Pathways Revealed by Force Spectroscopy and Steered Molecular Dynamics Simulations. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012, 134, 10428–10435.
Zheng, P.; Takayama, S.-i. J.; Mauk, A. G.; Li, H. Hydrogen Bond Strength Modulates the Mechanical Strength of Ferric-Thiolate Bonds in Rubredoxin. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012, 134, 4124–4131.
Lv, S.; Cao, Y.; Li, H. Tandem Modular Protein-Based Hydrogels Constructed Using a Novel Two-Component Approach. LANGMUIR 2011, 28, 2269–2274.
Cao, Y.; Li, H. Dynamics of Protein Folding and Cofactor Binding Monitored by Single-Molecule Force Spectroscopy. BIOPHYSICAL JOURNAL 2011, 101, 2009–2017.
Cao, Y.; Li, Y. D.; Li, H. Enhancing the Mechanical Stability of Proteins through a Cocktail Approach. BIOPHYSICAL JOURNAL 2011, 100, 1794–1799.
Zheng, P.; Li, H. Direct Measurements of the Mechanical Stability of Zinc-Thiolate Bonds in Rubredoxin by Single-Molecule Atomic Force Microscopy. BIOPHYSICAL JOURNAL 2011, 101, 1467–1473.
Peng, Q.; Fang, J.; Wang, M.; Li, H. Kinetic Partitioning Mechanism Governs the Folding of the Third FnIII Domain of Tenascin-C: Evidence at the Single-Molecule Level. JOURNAL OF MOLECULAR BIOLOGY 2011, 412, 698–709.
Aioanei, D.; Lv, S.; Tessari, I.; Rampioni, A.; Bubacco, L.; Li, H.; Samorì, B.; Brucale, M. Single-Molecule-Level Evidence for the Osmophobic Effect. ANGEWANDTE CHEMIE 2011, 123, 4486–4489.
Zheng, P.; Cao, Y.; Li, H. Facile Method of Constructing Polyproteins for Single-Molecule Force Spectroscopy Studies. LANGMUIR 2011, 27, 5713–5718.
Zheng, P.; Li, H. Highly Covalent Ferric-Thiolate Bonds Exhibit Surprisingly Low Mechanical Stability. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011, 133, 6791–6798.
Zheng, P.; Cao, Y.; Bu, T.; Straus, S. K.; Li, H. Single Molecule Force Spectroscopy Reveals That Electrostatic Interactions Affect the Mechanical Stability of Proteins. BIOPHYSICAL JOURNAL 2011, 100, 1534–1541.
Cao, Y.; Li, H. Single-Molecule Force-Clamp Spectroscopy: Dwell Time Analysis and Practical Considerations. LANGMUIR 2011, 27, 1440–1447.
Zhuang, S.; Linhananta, A.; Li, H. Phenotypic Effects of Ehlers-Danlos Syndrome-Associated Mutation on the FnIII Domain of Tenascin-X. PROTEIN SCIENCE 2010, 19, 2231–2239.
Jollymore, A.; Li, H. Measuring “Unmeasurable” Folding Kinetics of Proteins by Single-Molecule Force Spectroscopy. JOURNAL OF MOLECULAR BIOLOGY 2010, 402, 610–617.
Li, H.; Cao, Y. Protein Mechanics: From Single Molecules to Functional Biomaterials. ACCOUNTS OF CHEMICAL RESEARCH 2010, 43, 1331–1341.
Lv, S.; Dudek, D. M.; Cao, Y.; Balamurali, M. M.; Gosline, J.; Li, H. Designed Biomaterials to Mimic the Mechanical Properties of Muscles. NATURE 2010, 465, 69–73.
Peng, Q.; Li, H. Domain Insertion Effectively Regulates the Mechanical Unfolding Hierarchy of Elastomeric Proteins: Towards Engineering Multi-Functional Elastomeric Proteins. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2009, 131, 14050–14056.
Peng, Q.; Li, H. Direct Observation of the Tug-of-War during the Folding of a Mutually Exclusive Protein. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2009, 131, 13347–13354.
Zhuang, S.; Peng, Q.; Cao, Y.; Li, H. Modulating the Mechanical Stability of Extracellular Matrix Protein Tenascin-C in a Controlled and Reversible Fashion. JOURNAL OF MOLECULAR BIOLOGY 2009, 390, 820–829.
Cao, Y.; Er, K. S.; Parhar, R.; Li, H. A Force-Spectroscopy-Based Single-Molecule Metal-Binding Assay. CHEMPHYSCHEM 2009, 10, 1450–1454.
Peng, Q.; Zhuang, S.; Wang, M.; Cao, Y.; Khor, Y.; Li, H. Mechanical Design of the Third FnIII Domain of Tenascin-C. JOURNAL OF MOLECULAR BIOLOGY 2009, 386, 1327–1342.
Jollymore, A.; Lethias, C.; Peng, Q.; Cao, Y.; Li, H. Nanomechanical Properties of Tenascin-X Revealed by Single Molecule Force Spectroscopy. JOURNAL OF MOLECULAR BIOLOGY 2009, 385, 1277–1286.
Balamurali, M. M.; Sharma, D.; Chang, A.; Khor, D.; Chu, R.; Li, H. Recombination of Protein Fragments: A Promising Approach toward Engineering Proteins with Novel Nanomechanical Properties. PROTEIN SCIENCE 2008, 17, 1815–1826.
Sharma, D.; Feng, G.; Khor, D.; Genchev, G. Z.; Lu, H.; Li, H. Stabilization Provided by Neighboring Strands Is Critical for the Mechanical Stability of Proteins. BIOPHYSICAL JOURNAL 2008, 95, 3935–3942.
Li, H. “Mechanical Engineering” of Elastomeric Proteins: Toward Designing New Protein Building Blocks for Biomaterials. ADVANCED FUNCTIONAL MATERIALS 2008, 18, 2643–2657.
Cao, Y.; Yoo, T.; Li, H. Single Molecule Force Spectroscopy Reveals Engineered Metal Chelation Is a General Approach to Enhance Mechanical Stability of Proteins. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2008, 105, 11152–11157.
Cao, Y.; Li, H. Engineering Tandem Modular Protein Based Reversible Hydrogels. CHEMICAL COMMUNICATIONS 2008, 35, 4144–4146.
Cao, Y.; Li, H. Engineered Elastomeric Proteins with Dual Elasticity Can Be Controlled by a Molecular Regulator. NATURE NANOTECHNOLOGY 2008, 3, 512–516.
Li, H.; Wang, H.-C.; Cao, Y.; Sharma, D.; Wang, M. Configurational Entropy Modulates the Mechanical Stability of Protein GB1. JOURNAL OF MOLECULAR BIOLOGY 2008, 379, 871–880.
Cao, Y.; Yoo, T.; Zhuang, S.; Li, H. Protein–Protein Interaction Regulates Proteins’ Mechanical Stability. JOURNAL OF MOLECULAR BIOLOGY 2008, 378, 1132–1141.
Cao, Y.; Kuske, R.; Li, H. Direct Observation of Markovian Behavior of the Mechanical Unfolding of Individual Proteins. BIOPHYSICAL JOURNAL 2008, 95, 782–788.
Peng, Q.; Li, H. Atomic Force Microscopy Reveals Parallel Mechanical Unfolding Pathways of T4 Lysozyme: Evidence for Kinetic Partitioning Mechanism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2008, 105, 1885–1890.
Cao, Y.; Li, H. How Do Chemical Denaturants Affect the Mechanical Folding and Unfolding of Proteins?. JOURNAL OF MOLECULAR BIOLOGY 2008, 375, 316–324.
Cao, Y.; Balamurali, M. M.; Sharma, D.; Li, H. A Functional Single-Molecule Binding Assay via Force Spectroscopy. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2007, 104, 15677–15681.
Sharma, D.; Perisic, O.; Peng, Q.; Cao, Y.; Lam, C.; Lu, H.; Li, H. Single-Molecule Force Spectroscopy Reveals a Mechanically Stable Protein Fold and the Rational Tuning of Its Mechanical Stability. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2007, 104, 9278–9283.
Li, H. Engineering Proteins with Tailored Nanomechanical Properties: A Single Molecule Approach. ORGANIC & BIOMOLECULAR CHEMISTRY 2007, 5, 3399–3406.
Cao, Y.; Li, H. Polyprotein of GB1 Is an Ideal Artificial Elastomeric Protein. NATURE MATERIALS 2007, 6, 109–114.
Sharma, D.; Cao, Y.; Li, H. Engineering Proteins with Novel Mechanical Properties by Recombination of Protein Fragments. ANGEWANDTE CHEMIE INTERNATIONAL EDITION 2006, 45, 1–7.
Cao, Y.; Li, H. Single Molecule Force Spectroscopy Reveals a Weakly Populated Microstate of the FnIII Domains of Tenascin. JOURNAL OF MOLECULAR BIOLOGY 2006, 361, 372–381.
Cao, Y.; Lam, C.; Wang, M.; Li, H. Nonmechanical Protein Can Have Significant Mechanical Stability. ANGEWANDTE CHEMIE INTERNATIONAL EDITION 2006, 45, 642–645.
Wang, M.; Cao, Y.; Li, H. The Unfolding and Folding Dynamics of TNfnALL Probed by Single Molecule Force-Ramp Spectroscopy. POLYMER 2006, 47, 2548–2554.
Fernandez, J. M.; Li, H. Force-Clamp Spectroscopy Monitors the Folding Trajectory of a Single Protein. SCIENCE 2004, 303, 1674–1678.
Marszalek, P. E.; Oberhauser, A. F.; Li, H.; Fernandez, J. M. The Force-Driven Conformations of Heparin Studied with Single Molecule Force Microscopy. BIOPHYSICAL JOURNAL 2003, 85, 2696–2704.
Carrion-Vazquez, M.; Li, H.; Lu, H.; Marszalek, P. E.; Oberhauser, A. F.; Fernandez, J. M. The Mechanical Stability of Ubiquitin Is Linkage Dependent. NATURE STRUCTURAL BIOLOGY 2003, 10, 738–743.
Li, H.; Fernandez, J. M. Mechanical Design of the First Proximal Ig Domain of Human Cardiac Titin Revealed by Single Molecule Force Spectroscopy. JOURNAL OF MOLECULAR BIOLOGY 2003, 334, 75–86.
Linke, W. A.; Kulke, M.; Li, H.; Fujita-Becker, S.; Neagoe, C.; Manstein, D. J.; Gautel, M.; Fernandez, J. M. PEVK Domain of Titin: An Entropic Spring with Actin-Binding Properties. JOURNAL OF STRUCTURAL BIOLOGY 2002, 137, 194–205.
Li, H.; Linke, W. A.; Oberhauser, A. F.; Carrion-Vazquez, M.; Kerkvliet, J. G.; Lu, H.; Marszalek, P. E.; Fernandez, J. M. Reverse Engineering of the Giant Muscle Protein Titin. NATURE 2002, 418, 998–1002.
Marszalek, P. E.; Li, H.; Fernandez, J. M. Fingerprinting Polysaccharides with Single Molecule Atomic Force Microscopy. NATURE BIOTECHNOLOGY 2001, 19, 258–262.
Li, H.; Oberhauser, A. F.; Redick, S. D.; Carrion-Vazquez, M.; Erickson, H. P.; Fernandez, J. M. Multiple Conformations of PEVK Proteins Detected by Single Molecule Techniques. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2001, 98, 10682–10686.
Marszalek, P. E.; Greenleaf, V. J.; Li, H.; Oberhauser, A. F.; Fernandez, J. M. AFM Captures Quantized Plastic Deformation in Gold Nanowires. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2000, 97, 6282–6286.
Li, H.; Zhang, W. K.; Xu, W. Q.; Zhang, X. Hydrogen Bonding Governs the Elastic Properties of Poly(vinyl alcohol) in Water: Single-Molecular Force Spectroscopic Studies on PVA. MACROMOLECULES 2000, 33, 465–469.
Li, H.; Oberhauser, A. F.; Fowler, S.; Clarke, J.; Fernandez, J. M. Atomic Force Microscopy Reveals the Mechanical Design of a Modular Protein. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 2000, 97, 6527–6531.
Li, H.; Carrion, M.; Oberhauser, A. F.; Marszalek, P. E.; Fernandez, J. M. Point Mutations Alter the Mechanical Properties of Immunoglobulin Modules. NATURE STRUCTURAL BIOLOGY 2000, 7, 1117–1120.
Li, H.; Rief, M.; Oesterhelt, F.; Gaub, H. Force Spectroscopy on Single Xanthan Molecules. APPLIED PHYSICS A: MATERIALS SCIENCE & PROCESSING 1999, 68, 407–410.
Marszalek, P. E.; Pang, Y. P.; Li, H.; Oberhauser, A.; Fernandez, J. M. Atomic Levers Control Pyranose Ring Conformations. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA 1999, 96, 7894–7898.
Li, H.; Liu, B.; Zhang, X.; Gao, C.; Shen, J.; Zou, G. Single Molecule Force Spectroscopy on Polyacrylic Acid. LANGMUIR 1999, 15, 2120–2124.
Li, H.; Rief, M.; Oesterhelt, F.; Gaub, H.; Zhang, X.; Shen, J. Single Molecule Force Spectroscopy on 1,4-Linked Polysaccharides. CHEMICAL PHYSICS LETTERS 1999, 305, 197–201.
Marszalek, P. E.; Lu, H.; Li, H.; Carrion-Vazquez, M.; Oberhauser, A. F.; Fernandez, J. M. Mechanical Unfolding Intermediates in Titin Modules. NATURE 1999, 402, 100–103.
Li, H.; Zhang, W.; Zhang, X.; Shen, J.; Liu, B.; Gao, C.; Zou, G. Single Molecule Force Spectroscopy on Polyvinyl Alcohol. MACROMOLECULAR RAPID COMMUNICATIONS 1998, 19, 609–611.
Li, H.; Wang, Z.; Zhao, B.; Xiong, H.; Zhang, X.; Shen, J. C. Well Ordered Structure and Phase Transition Behaviors of a Reversed Duckweed Polymeric LB Film Studied by FTIR and SAXD. LANGMUIR 1998, 14, 423–428.
Li, H.; Rief, M.; Oesterhelt, F.; Gaub, H. Single Molecule Force Spectroscopy on Xanthan by AFM. ADVANCED MATERIALS 1998, 10, 316–319.
Li, H.; Zhang, X.; Zhang, R.; Shen, J.; Zhao, B.; Xu, W. Thermotropic Liquid Crystals Behaviors of an Amphiphilic Polymer Not Bearing Mesogens. MACROMOLECULE 1995, 28, 8178–8181.